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Figure 3 | Virology Journal

Figure 3

From: Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus

Figure 3

Confirmation of siRNA suppression of host protein expression and reduction of DENV infection. Gene specific siRNA against (A) CLTC, (B) AP2B1, (C) DNM2, (D) ARRB1, (E) ATP6V0A1 and (F) ARPC1B were reverse-transfected into Huh7 cells at different concentrations (0 nM to 50 nM) and subjected to DENV infection. Dosage dependent inhibition of DENV infection can be observed for these selected genes. At the same time, Western blots were performed after treatment with siRNAs to ensure the knockdown of the specific protein expression. Dosage-dependent reduction of protein expression is also observed for the indicated genes corresponding to the concentrations of the transfected siRNA. The blots are also re-probed with β-actin-specific antibody which served as a gel-loading control (lower panels). (G) Deconvolution of siRNA smartpools that reduced infectious entry of DENV. The experiments shown were repeated with the deconvoluted siRNA sequences (4 individual siRNA) from the Smartpool. The data were displayed for 3 independent experiments.

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Virology Journal

ISSN: 1743-422X