Figure 8

Effects of overexpression of HDAC4 mutants on integrase-dependent apoptosis in ATM-deficient cells. Control HeLa cells and HeLa cells overexpressing HDAC4 mutants were infected at m.o.i. 2 and treated with the ATM inhibitor (10 μM). One day post-infection, cells were harvested, lysed and cell lysates subjected to western blotting with an anti-PARP antibody. M - uninfected HeLa cells, C1 - cells transfected with the control EGFP expressing PEGFP-C1 plasmid and infected with the vector, 1061 - cells expressing the HDAC4-1061 mutant and infected with the vector, 1084 - cells expressing the HDAC4-1084 protein and infected with the vector. KU - the ATM inhibitor. N - normal PARP protein, A - the 85-kDa fragment of PARP, which is an apoptotic marker. GAPDH served as a loading control.