Skip to main content

Advertisement

Figure 5 | Virology Journal

Figure 5

From: Transcription phase, protein characteristics of DEV UL45 and prokaryotic expression, antibody preparation of the UL45 des-transmembrane domain

Figure 5

Expression and purification of recombinant protein. M1: Protein Marker; 1: Total protein from pET-32a (+) after induction; 2: Total protein from recombinant plasmid PET32a(+)-UL45Δ after induction; 3: Uninduced control; 4: The clear supernatant after ultrasonic disruption; 5: Purified recombinant UL45Δ protein using a single chromatographic step of IMAC on Ni 2+ -NTA agarose.

Back to article page