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Table 1 Yields Obtained for Influenza Virus Grown in Baggeda vs Non-bagged ECEa.

From: Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs

  Bagged ECE Non-bagged ECE
Virus Strain TCID 50 b EID c TCID 50 EID
A/NWS/1933 (H1N1) 8.05 ± 0.15 9.25 ± 0.25 8.1 ± 1.0 9.4 ± 0.2
A/HK/8/1968 (H3N2) 7.95 ± 0.05 9.3 ± 1.0 7.75 ± 0.25 9.0 ± 0.2
  1. aCAS fluids from four ECE were harvested and pooled 48 hrs after infection of 9-day old ECE with 102 TCID50 units of virus and incubation at 34°C. The experiment was repeated three separate times. bLog10 of TCID50/ml in MDCK cells in serum-free growth medium plus trypsin. cLog10 of EID50/ml obtained in non-bagged 10-day-old ECE incubated at 34°C.