Gas-permeable ethylene bags for the small scale cultivation of highly pathogenic avian influenza H5N1 and other viruses in embryonated chicken eggs

Table 1 Yields Obtained for Influenza Virus Grown in Bagged^a vs Non-bagged ECE^a.

^aCAS fluids from four ECE were harvested and pooled 48 hrs after infection of 9-day old ECE with 10² TCID₅₀ units of virus and incubation at 34°C. The experiment was repeated three separate times. ^bLog₁₀ of TCID₅₀/ml in MDCK cells in serum-free growth medium plus trypsin. ^cLog₁₀ of EID₅₀/ml obtained in non-bagged 10-day-old ECE incubated at 34°C.

ISSN: 1743-422X