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Table 3 Reproducibility of the real-time PCR/RT-PCR assay

From: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

Reproducibility

XMRV DNA/RNA (copies/μl/IU/ml)

Number of determinations

Mean Ct (DNA/RNA)

SD (DNA/RNA)

CV(%) (DNA/RNA)

within-run

105

10

24.47/25.13

0.50/0.52

2.03/2.06

 

104

10

27.25/27.99

0.48/0.48

1.76/1.70

 

102

10

33.81/34.62

0.94/0.90

2.80/2.59

between-run

105

10

24.97/26.17

0.28/0.32

1.14/1.23

 

104

10

27.98/29.14

0.30/0.31

1.07/1.06

 

102

10

34.43/35.94

0.88/0.99

2.56/2.74