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Table 1 Primer and probe sequences

From: Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

Primer or probe

Sequence (5'-3')

Gag-1S

5'-TTGGCCGGCC ACATGAGGATCACCCATGTCGTGTTCCCAATAAAGCCTTTTGCTGTTTG-3'

Gag-1A

5'-ATTCAGACGGGGGCGGGAATGTCGGCTTTGAGGGGGCCTGAGTGTCTCTGTCTCTCGTC-3'

Gag-2S

5'-GACGAGAGACAGAGACACTCAGGCCCCCTCAAAGCCGACATTCCCGCCCCCGTCTGAAT-3'

Gag-2A

5'-GAGTGATCTATGGTGGAGACATGGGTGATCCTCATGTGCCGCCTCTTCTTCATTG-3'

HCV- S

5'-CAATGAAGAAGAGGCGGCACATGAGGATCACCCATGTCTCCACCATAGATCACTC-3'

HCV-A

5'-CCTTAATTAA ACATGGGTGATCCTCATGTGGTTGGTGTTACGTTTGGTT-3'

Gag-3S

5'-GGACTTTTTGGAGTGGCTTTGTT-3'

Gag-3A

5'-GCGTAAAACCGAAAGCAAAAAT-3'

Gag p

FAM5'-ACAGAGACACTTCCCGCCCCCG-3'BHQ

IC p

HEX5'-CAGGCCCCCTCAAAGCCGACAT-3'BHQ

β-actin A

5'-CCTGGCACCCAGCACAAT-3'

β-actin S

5'-GCTGATCCACATCTGCTGGAA-3'

β-actin p

FAM5'-ATCAAGATCATTGCTCCTCCTGAGCGC-3'TAKARA

  1. Fse I and Pac I restriction enzyme sites are underscored; a C-variant pac site is indicated by boldface type. The broken line indicates the internal control sequences inserted in the CIC. FAM: 6-carboxyfluorescein; HEX: 6-carboxyhexachlorofluorescein; BHQ: Black hole quencher dye.
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Virology Journal

ISSN: 1743-422X