Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome

Table 1 Primer and probe sequences

Primer or probe	Sequence (5'-3')
Gag-1S	5'-TTGGCCGGCC ACATGAGGATCACCCATGTCGTGTTCCCAATAAAGCCTTTTGCTGTTTG-3'
Gag-1A	5'-ATTCAGACGGGGGCGGGAATGTCGGCTTTGAGGGGGCCTGAGTGTCTCTGTCTCTCGTC-3'
Gag-2S	5'-GACGAGAGACAGAGACACTCAGGCCCCCTCAAAGCCGACATTCCCGCCCCCGTCTGAAT-3'
Gag-2A	5'-GAGTGATCTATGGTGGAGACATGGGTGATCCTCATGTGCCGCCTCTTCTTCATTG-3'
HCV- S	5'-CAATGAAGAAGAGGCGGCACATGAGGATCACCCATGTCTCCACCATAGATCACTC-3'
HCV-A	5'-CCTTAATTAA ACATGGGTGATCCTCATGTGGTTGGTGTTACGTTTGGTT-3'
Gag-3S	5'-GGACTTTTTGGAGTGGCTTTGTT-3'
Gag-3A	5'-GCGTAAAACCGAAAGCAAAAAT-3'
Gag p	FAM5'-ACAGAGACACTTCCCGCCCCCG-3'BHQ
IC p	HEX5'-CAGGCCCCCTCAAAGCCGACAT-3'BHQ
β-actin A	5'-CCTGGCACCCAGCACAAT-3'
β-actin S	5'-GCTGATCCACATCTGCTGGAA-3'
β-actin p	FAM5'-ATCAAGATCATTGCTCCTCCTGAGCGC-3'TAKARA

Fse I and Pac I restriction enzyme sites are underscored; a C-variant pac site is indicated by boldface type. The broken line indicates the internal control sequences inserted in the CIC. FAM: 6-carboxyfluorescein; HEX: 6-carboxyhexachlorofluorescein; BHQ: Black hole quencher dye.