Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus

Liu, Xiaoli; Han, Zongxi; Shao, Yuhao; Yu, Dan; Li, Huixin; Wang, Yu; Kong, Xiangang; Liu, Shengwang

doi:10.1186/1743-422X-7-223

Virology Journal

Table 1 Sequences of the primers used in this study

From: Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus

Fragments	Primer sequences (5' - 3')^a		Position in UL26 gene^b	Size of amplicon (bp)
	Sense	Negative sense
UL26c	GGATCC ATG CAATCTACTATGACG	GTCGAC TCA ACATCTATTACACATCA	1042-2124	1083
F1	GGATCC ATG CAATCTACTATGACG	GTCGAC TTA CAGCTGCCCTCCCTGGAC	1042-1347	306
F2	GGATCC ATG TATGGACAGCCTGTTTAT	GTCGAC TTA AGCTAATGGTCCAGTAGA	1294-1731	438
F3	GGATCC ATG CCTACTGGACAAGGTAAC	GTCGAC TCA ACATCTATTACACATCA	1681-2124	444
F2-1	GGATCC ATG TATGGACAGCCTGTTTAT	CTTTGGTCGAC TTA ATCTCCAGATTCGACGGC	1294-1455	162
F2-2	TGCAGGGATCC ATG GCAATTGCTGCAGATAGG	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1411-1575	165
F2-3	TGCAGGGATCC ATG AATGACGACCAGTTAGAT	GTCGAC TTA AGCTAATGGTCCAGTAGA	1531-1731	201
F4	TGCAGGGATCC ATG AATGACGACCAGTTAGAT	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1531-1575	45
F5	TGCAGGGATCC ATG GACGACCAGTTAGATGGT	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1534-1575	42
F6	TGCAGGGATCC ATG CAGTTAGATGGTGACAAT	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1540-1575	36
F7	TGCAGGGATCC ATG TTAGATGGTGACAATATC	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1543-1575	33
F8	TGCAGGGATCC ATG GATGGTGACAATATCTAT	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1546-1575	30
F9	TGCAGGGATCC ATG GGTGACAATATCTATTAT	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1549-1575	27
F10	TGCAGGGATCC ATG GACAATATCTATTATCCG	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1552-1575	24
F11	TGCAGGGATCC ATG AATATCTATTATCCGGGG	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1555-1575	21
F12	TGCAGGGATCC ATG ATCTATTATCCGGGGGAA	CTTTGGTCGAC TTA TTCCCCCGGATAATAGAT	1558-1575	18
F13	GATCC ATG ATCTATTATCCGGGGTAA G	TCGAC TTA CCCCGGATAATAGATCAT G	1558-1572	15
F14	GATCC ATG TATTATCCGGGGGAATAA G	TCGAC TTA TTCCCCCGGATAATACAT G	1561-1575	15

^a The introduced restriction enzyme sites (Bam HI and Sal I) in each primer are underlined. The stop codon, TCA, of the DEV UL26 gene and the introduced start and stop codons in the primers of the UL26 fragments are shown in bold, respectively.
^b The nucleotide positions correspond to those of the DEV UL26 gene, GenBank accession no. EF203709.

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ISSN: 1743-422X

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