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Figure 3 | Virology Journal

Figure 3

From: Identification of a novel linear B-cell epitope in the UL26 and UL26.5 proteins of Duck Enteritis Virus

Figure 3

Precise localization of the epitope defined by mAb 1C8. The reactivity of mAb 1C8 with different truncated recombinant proteins was determined by western blotting and ELISA. The names of the proteins are the same as in Table 1. Recombinant GST (rGST) protein was used as a negative control in both the western blotting and indirect ELISA. A) The western blotting results of mAb 1C8 with peptides F1, F2, F3, F2-1, F2-2 and F2-3. B) The western blotting results of mAb 1C8 with peptides F4, F5, F6, F7, F8, F9 and F10. C) The western blotting results of mAb 1C8 with peptides F11, F12, F13 and F14. D) The results of ELISA of mAb 1C8 with the 17 recombinant proteins. The pink columns indicated the results of the ELISA of mAb 1C8 with the 17 recombinant proteins and the green columns are negative controls, which showed the results of ELISA of SP2/0 cell culture media with the recombinant proteins.

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