Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Substitution of adeno-associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences

Figure 2

Packaging comparisons between the wild-type and modified viruses. (A) Dot blot analysis of 3-fold serial dilutions of nuclease-resistant AAV2 DNA made using pSub201(-) (wt), pVM108 (108) pVM112 (112) or pVM141 (141). The first dot in each row has the nuclease-resistant DNA obtained from 5 μl of virus supernatant. (B) Standards. The indicated amount of linearized and denatured pSub201(-) was applied to the same membrane. All samples were probed with random primer radiolabeled AAV2 DNA.

Back to article page

Virology Journal

ISSN: 1743-422X