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Figure 3 | Virology Journal

Figure 3

From: Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element

Figure 3

The effect of pShRNA PRE 1317-1337 on the expression of cccDNA. Bar graphs indicate mean values of thresholds of three independent experiments. In this study, HepG2 cells were transiently transfected in triplicate with 45 ng of the pSpliceLuc/fPRE, 5 ng of the phRL-SV40 and different amounts of the pShRNA constructs as indicated. pSilencer-Negative was used to make up total DNA plasmid to 350 ng. Cells were harvested and analyzed for luciferase activity and western blot analysis after 48 h of incubation. '***' indicates significant differences of comparative threshold comparing to the controls (positive cccDNA and cells without transfection of pShPRE 1317-1337) with p < 0.001 (by t-test).

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