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Figure 3 | Virology Journal

Figure 3

From: Upregulation of a novel eukaryotic translation initiation factor 5A (eIF5A) in dengue 2 virus-infected mosquito cells

Figure 3

Validation of the eIF5A gene expression level in C6/36 cells with Den-2 virus infection. RNAs extracted from C6/36 cells with mock infection (Mock), UV-inactivated Den-2 virus (UV), or intact Den-2 virus (Den-2) at a multiplicity of infection (MOI) of 1 were evaluated by a quantitative real-time RT-PCR assay. The quantitative real-time PCR analysis of eIF5A was monitored and normalized to the expression of 18S, which was used as an internal control. Ratios of the normalized expressions of eIF5A of Den-2-infected cells were relative to that of mock-infected cells. The results showed that the expression of eIF5A was significantly higher in the group with Den-2 virus infection (p < 0.05), but not in those inoculated with UV-inactivated Den-2 virus (p > 0.05).

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Virology Journal

ISSN: 1743-422X