Figure 4

Effects of amantadine with or without interferon on HAV KRM003 genotype IIIB strain replication in African green monkey kidney cell GL37. GL37 cell monolayers in 96-well culture plates were infected with HAV [at a multiplicity of infection (MOI) of 5 or 40] for 1 h at 37°C in a CO₂ incubator. Amantadine and/or IFN was added to cells. After the incubation for 72 h, infected cells were evaluated with ELISA. The rate of virus survival was measured using this equation: Virus survival rate (%) = 100 × Absorbance with drug/Absorbance without drug.