Figure 6

Bioluminescent imaging analysis of mice infected with an adenovirus containing a capsid-incorporated Luc protein or expressing Luc protein within the E3 region. Athymic nude mice were implanted on both flanks with subcutaneous xenografts of A549 cells. After tumor formation, mice were injected intratumorally with Ad-wt-pIX-Luc in the right tumor nodule and with Ad-wt-E3-Luc in the left tumor nodule. Mice were injected with D-Luciferin prior to imaging; images were captured over a 15 day period. Values are expressed as mean ± standard deviation of values obtained from 7 mice. Regions of interest were drawn around the tumor and the total counts (photons) were summed in the tumor. The total counts in each region of interest were normalized to total acquisition time to obtain counts/sec. The tumors depicted in this figure are a representative of one mouse in the study. The instrument used is the IVIS Xenogen with Living image 3.1 software. Longitudinal analysis was performed using mixed models repeated measures analysis. The model used in this analysis included terms for luciferase group (Ad-wt-pIX-Luc, Ad-wt-E3-Luc), time (Day), and the interaction between luciferase group and time. Cross-sectional analyses for luciferase group comparisons were performed using the paired t-test (where the analyses included only mice that have Ad-wt-pIX-Luc and Ad-wt-E3-Luc measurements), and the two-group t-test (where the analyses included data for all mice but do not account for the fact that data on different days come from the same mice) or the two-group t-test assuming unequal variances when needed. All statistical tests were two-sided and were performed using a significance level of 5% (i.e. alpha = 0.05). Statistical analyses were performed using SAS (version 9.1.3; SAS Institute, Inc., Cary, NC).