Figure 5
From: Evaluation of adenovirus capsid labeling versus transgene expression
In vitro analysis of luciferase activity of an adenovirus with a capsid-incorporated Luc protein or E3 expressed Luc protein. A549 cells were infected with 100 IP/cell of non-replicative Ad, Adwt, Ad-wt-pIX-Luc, or Ad-wt-E3-Luc viruses. At 24, 48, or 72 h.p.i., the cells were harvested and the cell lysates were collected and subjected to protein quanitation and luciferase assay. Values are expressed as mean ± standard deviation of three replicates. The asterisks (**) indicates a P value = 0.001. Comparisons were performed using analysis of variance followed by the Tukey-Kramer multiple comparisons test.