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Figure 5 | Virology Journal

Figure 5

From: Serological characterization of guinea pigs infected with H3N2 human influenza or immunized with hemagglutinin protein

Figure 5

PepScan ELISA of serum pools from guinea pigs immunized with recombinant HA protein. Group serum pools (1:750 dilution) were analyzed for recognition of linear epitopes by reactivity to overlapping peptides bound to microtiter plates. The black bars indicate the magnitude of the ELISA reactivity as a measure of Optical Density (O.D.) for the recognition of specific peptides. Sequential bleeds were tested from the prebleed (A) and 3 (B), 5 (C), and 12 (D) weeks after the initial immunization. Reactivity to the peptides was compared between the three groups to identify potential linear epitopes. Group 1: mock immunized negative control group (left column), Group 3: lower dose HA-immunized (center), Group 4: higher dose HA-immunized (right column).

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