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Figure 4 | Virology Journal

Figure 4

From: A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

Figure 4

Immunological properties of the pcDNAHN176-construct. A) Detection of the HN176 polypeptide and HN protein by Western Blot. Lane 1) Negative control, uninfected cells and sera from pcDNAHN176-immunized rabbits; Lane 2) MuV-infected-Vero cells and anti-MuV serum; Lane 3) pcDNAHN176-transfected cells and anti-MuV serum; Lane 4) MuV-infected-Vero cells and sera from pcDNAHN176-immunized rabbits; Lane 5) pcDNAHN176-transfected cells and sera from pcDNAHN176-immunized rabbits. The upper arrow indicates in lane 2 and 4 the position of the complete viral HN protein, and the lower arrow indicates the position of the HN176 polypeptide in lanes 3 & 5. B) Body gain weight of hamsters immunized and challenged with MuV. Group A, animals without immunization and uninfected; Group B, viral control group (animals without immunization and challenged with MuV); Group C, animals immunized with pcDNA3.1 without challenge; Group D, animals immunized with pcDNAHN176-construct without challenge; Group E, animals immunized with pcDNA3.1 and challenged with MuV; Group F, animals immunized with pcDNAHN176 and challenged with MuV. C) Virus isolation from different organs of the hamsters groups. MuV was detected by HD, quantifying the amount of Hb. D) Detection of IL associated to Th1 response in the hamsters groups. E) Detection of IL associated to Th2 response in the hamsters groups. ILs were measured using the Luminex System (Invitrogen ®). F) Lymphoproliferation index of spleen cells obtained from the hamsters groups. Cell proliferation was measured by MTT method.

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