Skip to main content
Figure 2 | Virology Journal

Figure 2

From: A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

Figure 2

Expression of the pcDNAHN176-construct. A) Detection of HN176 mRNA in transfected Vero cells by RT-PCR amplification. Lane 1) ϕX174 DNA-HaeIII marker; Lane 2) RT/PCR negative control; Lane 3) Positive control (MuV-infected cells); Lane 4) Vero-untransfected cells, 5) Vero cells transfected with the plasmid pcDNA3.1, Lane 6) Vero cells transfected with the pcDNA-HN176-construct. The arrow indicates 580 bp amplicon. 1% agarose gel/100 V/1 hr/49 mA. B) RT-PCR Controls. Lane 1) ϕX174 DNA-HaeIII marker; Lane 2) PCR amplification of the RNA samples obtained from pcDNAHN176-transfected Vero cells without a previous RT reaction; Lane 3 and 4) RT/PCR amplification of β-actin gene using RNA samples obtained from HeLa cells and pcDNAHN176-transfected Vero cells, respectively. C) Immunodetection of the HN176 polypeptide by immunochemistry (1st row) and immunofluorescence (2nd row). Frames 1 & 5 mock infected cells; 2 & 6 pcDNA3.1-transfected cells; 3 & 7 MuV-infected cells; 4 & 8 pcDNAHN176-transfected cells, the blue (immunochemistry) and the green (immunofluorescence) colors indicate a positive reaction, N indicates the nucleus. 40×

Back to article page

Virology Journal

ISSN: 1743-422X