Table 1 Details of the primers used for PCR amplification in the study.
Primer Name | Sequence (5'→3'); location with respect to S27 sequence (GenBank Accession AF369024) | Target | Ta (°C) | Amplicon size | Reference |
|---|---|---|---|---|---|
RT PCR for CHIKV detection in patient and adult mosquitoes derived from larvae | |||||
E1 F | tacccatttatgtggggc (10246-10263) | Â | 52 | 294bp | [16] |
E1 R | gcctttgtacaccacgatt (10539-10521) | E1 | Â | Â | Â |
NSP2F | tgccatgggaataatagagactccg (1682-1699) | Â | Â | Â | Â |
ChR6 | gcgagtcaaccgtacgtgcag (2390-2370) | nsP2 | 55 | 709bp | This study |
ChF27 | gtcccctaagagacacattg (11486-11505) | Â | Â | Â | Â |
ChR28 | tacgtccctgtgggttcggagaat (11798-11780) | 3'NTR | 52 | 313bp | [14] |
RT PCR of partial sequences CHIKV genes for sequencing and phylogenetic analysis | |||||
E1Fseq1 | gctccgcgtcctttacc (10389-10405) | Â | Â | Â | Â |
E1Rseq1 | atggcgacgcccccaaagtc (10943-10924) | E1 | 55 | 555bp | This study |
ChF21 | gggacacttcatcctggc (8832-8849) | Â | Â | Â | [14] |
ChR22 | acatttgccagcggaaac (9332-9315) | E2 | 55 | 501bp | Â |
ChF8 | cctatcctcgaaacagcg (3134-3151) | Â | Â | Â | [14] |
ChR9 | gtgactctcttagtaggc (3636-3619) | nsP2 | 45 | 503bp | Â |