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Table 1 Summary of the results: UUKV-N protein point mutants in the M2 H, minigenome, and immunofluorescence assays.

From: Oligomerization of Uukuniemi virus nucleocapsid protein

UUKV-N protein
point mutants
M2H*
% of interaction
  Minigenome VLP IFA
Wt N protein 100 +++ +++ +++ +++
W7A > 100 (3) +++ - - +++
F10A 34 ± 4 (2) + + + +++
I14A 60 ± 18 (3) ++ - - +
W19A > 100 (3) +++ +/++ - +
I24A 56 ± 12 (2) ++ - - +
F27A > 100 (3) +++ - - ++
F31A 23 ± 5 (2) + - - ++
Y33A > 100 (2) +++ +++ +++ +++
QQ244,245AA > 100 (2) +++ +++ +++ +++
R251A 96 ± 11 (2) +++ +++ +++ +++
  1. * Full-length N-N protein interaction (100%, +++) was compared to the N-N interaction of point mutants, range from not affected (+++) to reduced (++) and to substantially reduced interaction (+). Each test was performed in triplicates, number of repetitions for each test is given in parentheses.
  2. In minigenome and VLP systems the CAT expression was either non-affected (+++), reduced (++), substantially reduced (+), or completely inhibited (-).
  3. Point mutants forming aggregates resembling full-length N protein (+++), showing microgranular (++) or diffuse (+) fluorescence pattern.