Oligomerization of Uukuniemi virus nucleocapsid protein

Table 1 Summary of the results: UUKV-N protein point mutants in the M2 H, minigenome, and immunofluorescence assays.

UUKV-N protein point mutants	M2H* % of interaction		Minigenome^†	VLP^†	IFA^‡
Wt N protein	100	+++	+++	+++	+++
W7A	> 100 (3)	+++	-	-	+++
F10A	34 ± 4 (2)	+	+	+	+++
I14A	60 ± 18 (3)	++	-	-	+
W19A	> 100 (3)	+++	+/++	-	+
I24A	56 ± 12 (2)	++	-	-	+
F27A	> 100 (3)	+++	-	-	++
F31A	23 ± 5 (2)	+	-	-	++
Y33A	> 100 (2)	+++	+++	+++	+++
QQ244,245AA	> 100 (2)	+++	+++	+++	+++
R251A	96 ± 11 (2)	+++	+++	+++	+++

* Full-length N-N protein interaction (100%, +++) was compared to the N-N interaction of point mutants, range from not affected (+++) to reduced (++) and to substantially reduced interaction (+). Each test was performed in triplicates, number of repetitions for each test is given in parentheses.
^† In minigenome and VLP systems the CAT expression was either non-affected (+++), reduced (++), substantially reduced (+), or completely inhibited (-).
^‡ Point mutants forming aggregates resembling full-length N protein (+++), showing microgranular (++) or diffuse (+) fluorescence pattern.

ISSN: 1743-422X