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Figure 3 | Virology Journal

Figure 3

From: 3'-coterminal subgenomic RNAs and putative cis-acting elements of Grapevine leafroll-associated virus 3 reveals 'unique' features of gene expression strategy in the genus Ampelovirus

Figure 3

RACE analysis of 5'NTR of GLRaV-3. (a) The schematic diagram showing the locations of primers used and expected size of amplicons and (b) agarose gel showing virus-specific DNA fragments (shown by arrow head on the right) amplified from cDNA made using primer AR. Lane 1 shows 883 bp fragment amplified with primers AAP and M1012 and lane 2 shows 1059 bp fragment amplified with primers AAP and M1013 primers. Lane M shows 1kb plus DNA marker (Invitrogen) for estimating the size of amplified DNA fragment. The size of marker DNA bands is indicated to the left. See Materials and methods for primer details.

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