Figure 3

Effect of increasing HIV-1 MOIs on integration levels in infected wt and LEDGF/p75-knockdown HeLa P4 cells. HeLa P4 and HeLaP4/shp75Cl15 (LEDGF/p75-knockdown) cells were incubated with or without 100 μM INS and infected at the indicated MOIs with (A) WT or (B) ΔRev HIV-1. The average number of integration events per cell was estimated as described in Methods. Black shading and dark grey shading are infected LEDGF/p75-knockdown cells without or with INS treatment, respectively; light grey shading and white shading are infected HeLa P4 cells without or with INS treatment, respectively. AZT was used at 2 μM concentration.