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Table 2 Highest trans-complementation-mediated enhancement of GFP expression of day 2 experiment

From: Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro

 

Virus

WtAd5

AdΔEP-TETP

AdCMV-lacZ

Cell line

MFI expression AdCMV-eGFP MOI 0.37

Highest fold enhancement

Input MOI AdCMV- eGFP of highest fold enhancement

Ratio WtAd5/AdCMV-eGFP

Highest fold enhancement

Input MOI AdCMV- eGFP of highest fold enhancement

Ratio

AdΔEP-TETP/AdCMV-eGFP

Highest fold enhancement

Input MOI AdCMV- eGFP of highest fold enhancement

Ratio

AdCMV-lacZ/AdCMV-eGFP

HeLa (cervical carcinoma)

9.0

7.2

0.37

81

2.8

1.1

81

1.2

1.1/3.3

81/9

SW480 (colon carcinoma)

2.9

133

0.37

243

18.3

1.1

81

1.1

3.3

9

A549 (lung carcinoma)

7.4

25.1

3.3

3

6.9

1.1

81

nd

  

DLD-1 (colon carcinoma)

15

7.7

3.3

9

3.8

0.37

243

nd

  

M000301 (melanoma)

3.3

19.1

1.1

81

28.8

0.37

243

3.3

0.37

243

M21L4 (melanoma)

3.3

11.2

0.37

243

13

1.1

81

1.5

1.1

27

SK-Mel23 (melanoma)

1.62

6.4

3.3

27

15.4

1.1

27

nd

  

MeWo (melanoma)

54

3.1

0.37

243

3.5

1.1

27

nd

  
  1. Nd: not determined