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Figure 4 | Virology Journal

Figure 4

From: Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro

Figure 4

Concentration-dependence and kinetics of Ad co-replication-mediated enhancement of eGFP expression. SW480 cells were infected with AdCMV-eGFP at MOIs of 0.37, 1.1, 3.3 and 10 alone (-), or in combination with wtAd5 at concentrations ranging from MOI 0.37 to 90, using serial 3-fold increases. The resulting ratios of wtAd5/AdCMV-eGFP are indicated. eGFP expression analyses were performed at day 1, 2, 3 and 4 p.i. by flow cytometry, and enhancement factors were calculated, following subtraction of auto-fluorescence of uninfected cells (cells only, co), unless, differences between uninfected and infected cells at MOI 0.37 were statistically not significant (not determined, nd).

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