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Figure 1 | Virology Journal

Figure 1

From: Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro

Figure 1

Structures of E1A promoter of wt Ad5, AdΔEP-TETP and E1 region of AdCMV-eGFP, AdTETP-CD40L/IL-2. (A) The first 650 bp of the wt Ad genome comprising the left ITR, enhancer elements (EI, EII), packaging elements (I-VII), minor (thin arrows) and major (bold arrow) transcriptional start sites, TATA-box and the beginning of the E1A ORF. (B) Insertion of TETP in combination with duplication of packaging elements V-VII and deletion of endogenous E1A promoter resulting in AdΔEP-TETP. (C) In AdCMV-eGPF, the deleted E1 region from nt 449 to 3333 is replaced with the CMV-eGPF expression cassette. (D) In AdTETP, the deleted E1 region from nt 449 to 3333 is replaced with the TETP-CD40L-IRES-IL-2 expression cassette. The nucleotide numbers refer to the corresponding wt sequence.

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Virology Journal

ISSN: 1743-422X