Figure 6
Specificity and sensitivity of the indirect ELISA. A. Specificity of the indirect ELISA. The different sera samples of duck anti-DEV, duck anti-DHV, duck anti-AI, duck anti-DVSHD, duck anti-R.A., duck anti-E. coli and duck anti-S.E. were tested by the indirect ELISA. Columns represent the mean absorbance from triplicate wells. All OD450 values except for DEV were lower than the cutoff value (1.0103). B. Sensitivity of the indirect ELISA. Different concentrations of the serum sample (from 1:10 dilution to 1:5120 dilution). At last the minimum detection limit of the duck anti-DEV positive sera was 1:2560, which could be detected by the indirect ELISA.