Skip to main content
Figure 3 | Virology Journal

Figure 3

From: Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis

Figure 3

The construction of expression plasmids were identified by colony PCR, single restriction enzyme Hind III digestion and two restriction enzymes ( Hind III and Xho I) digestion. M1, DNA marker-DL15000; M2, DNA MarkerIII. A. Identification of the expression plasmid pET-32b(+)/fgK. Lane 1, the colony PCR; Lane 2, two fragments after digesting the recombinant DNA with restriction enzymes Hind III and Xho I; Lane 3, one band by single restriction enzyme Hind III digestion. B. Identification of the expression plasmid pET-32b(+)/tgK. Lane 1, one band by single restriction enzyme Hind III digestion; Lane 2, two fragments after digesting the recombinant DNA with restriction enzymes Hind III and Xho I; Lane 3, the colony PCR.

Back to article page

Virology Journal

ISSN: 1743-422X