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Figure 3 | Virology Journal

Figure 3

From: Novel mutation in YMDD motif and direct neighbourhood in a child with chronic HBV-infection and clinical lamivudine and adefovir resistance - a scholarly case

Figure 3

Alignment of quasispecies analysis. Sequence of amino acids 157 - 237 in the reverse transcriptase domain of HBV polymerase is shown. The mutations that are associated with lamivudine restistance are at position rt204. The mutations that resulted in a stop codon are highlighted in yellow. A further mutation is located at position rtL180 M The HBV polymerase was amplified from patient material and cloned into TOPO vector, which was transformed into E. coli. After purification of plasmid DNA, parts of the pol gene were sequenced as described above. Alignment was done using Vector NTi software.

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