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Table 2 Determination of provirus copy number and chimerism by real-time PCR

From: Serial bone marrow transplantation reveals in vivo expression of the pCLPG retroviral vector

 

Short term

 

Chimerisma

Copy number (BMC)b

Non-transduced

ND

NA

pCLeGFP

  

>95%

0.03-0.04

>95%

0.02-0.03

>95%

0.02-0.03

pCLPGeGFP

  

>95%

0.06-0.07

>95%

0.04

>95%

0.03

  1. a, Chimerism determined using gDNA isolated from BMC recovered from transplant recipients where amplification of a Y-chromosome sequence was compared to a standard curve
  2. b, copy number was determined by amplifying a viral sequence and comparing this to a standard curve. Note that provirus was not quantifiable by this assay in peripheral blood, spleen or thymus.
  3. ND, not determined
  4. NA, not applicable