Figure 1
From: Serial bone marrow transplantation reveals in vivo expression of the pCLPG retroviral vector
p53-dependent expression from pCLPGeGFP revealed in a hematopoietic cell line. (A) Schematic representation of the parental, non-modified gamma retroviral vector, pCLeGFP, which utilizes the native LTR to drive transgene expression. For pCLPGeGFP, the LTR was modified by the removal of the enhancer region and insertion of a p53-responsive element, as described previously [8, 9]. (B) K562 cells were used to test expression of the pCLeGFP and pCLPGeGFP vectors in response to p53 activity (intensity of eGFP; au, arbitrary units). The data represent the mean and standard deviation of duplicate samples from 3 independent experiments.