Figure 7
From: Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
VP26 mutant virus shows a delayed proliferation. The recombinant virus HSV1-UG with a GFP-UL35 fusion gene was used to infect Vero or KMB-17 cells at 1 MOI. Punctated fluorescence spots were observed in the nucleus during infection, which is similar to the recombinant HSV1 with GFP-Vp16 fusion gene ([31]). At 12, 22, 24, 27, 36, 46, 60 and 72 h post-infection, samples of cell infected by HSV1-UG were collected and measured by real-time PCR with specific primers against α-4 gene. n = 3 for all time points. Error bars represent the standard error of the mean. Meanwhile, the KMB-17 cells infected by HSV1-UG were fixed with 5% glutaraldehyde and observed under the electron microscope. a. The Vero cells infected by VP26 mutant HSV1-UG were observed under fluorescence microscope at 12, 16 and 24 h. b. Growth curve of HSV1-UG compared with that of wild type HSV-1 in Vero cells. c. Electro-microscope observation of cells infected by HSV1-UG(X30, 000).