Figure 5

Reduction of CTMP-7 in cells by RNAi decreased the egress of HSV-1. KMB-17 cells transfected with pGE-Neg or pGE-CTMP plasmid were infected at an MOI of 1 with HSV-1 and incubated at 37°C. Viral loads (shown as delta Rn of virus DNA replication) of HSV-1 in KMB-17 cells were detected and analyzed by real-time PCR. At the indicated times post infection, samples of cell transfected and infected were harvested and extracted for further real-time PCR. The protocol is described in Material and Method. a. Viral loads shown with the copy of α-4, tk and gC genes of HSV1 at 12 hours post-infection in cells with CTMP-7 expression being inhibited. b. Viral loads shown with the copy of α-4, tk and gC genes of HSV1 at 24 hours post-infection in cells with CTMP-7 expression being inhibited. c. Viral loads shown with the copy of α-4, tk and gC genes of HSV1 at 36 hours post-infection in cells with CTMP-7 expression being inhibited.