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Figure 2 | Virology Journal

Figure 2

From: Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein

Figure 2

Intracellular analysis of CTMP-7 in HSV-1 infected cells. Vero cells were transiently transfected with plasmid pGFP-CTMP or pGFP-N followed by HSV-1 infection at 1 MOI, and then observed at 24 h post transfection by fluorescence microscopy without fixation. At 30 h post-infection, the cells were trypsinized, washed in PBS and analyzed by flow cytometry. a. Observation of Vero cells transfected with pGFP-CTMP or pGFP-N and infected by HSV1 at 24 h post-infection under fluorescence microscope. Upper row: Cells transfected with pGFP-CTMP and infected by HSV-I. Lower row: Cells transfected with pGFP-N and infected by HSV-I. b. Cytometric analysis of Vero cells transfected with pGFP-CTMP and pGFP-N followed by HSV-1 infection or not at 30 h post-infection. Column 1: Cells transfected with pGFP-N, upper - cells HSV-I infection free; lower - cells at 30 h post-infection. Column 2: Control cells transfected with pGFP-CTMP with HSV1 infection free, upper is cell at the same time point to 16 h post-infection of infective example, lower is cells at the same time point to 30 h post-infection of infective example. Column 3: Cells transfected with pGFP-CTMP and followed by HSV1 infection, upper is cell at 16 h post-infection; down is cell at 30 h post-infection.

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