Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages

Table 2 List of samples and PCR conditions in this study.

Isolate	Cell type	Date of transmission	Clones sequenced	Outer PCR primers	Outer PCR Temp.	Inner primers	Inner Temp.
314 serum	All	1/5/05	25	9.1a-9.2a	55	10.1a-10.2	60
314 T1	B cell	1/13/05	17	9.1a-9.2a	55	10.1a-10.2	60
314 T2	B cell	2/4/05	5	9.1a-9.2a	55	10.1a-10.2	60
384 serum	All	1/8/08	24	8 up-347 down	50	37 up-318 down	55
384 primary	Macrophage	1/30/08	26	8 up-347 down	55	37 up-318 down	55
384 P3HR1	B cell	1/30/08	25	9.1a-flap-9.2a-flap	50	10.1a-10.2	60
384 CEM	T cell	1/30/08	27	8 up-347 down	50	37 up-318 down	55
384 CB	Macrophage	4/7/08	8	8 up-347 down	55	37 up-318 down	55
388 serum	All	9/10/08	6	8 up-347 down	50	37 up-318 down	60
388 primary	Macrophage	9/12/08	5	8 up-347 down	50	37 up-318 down	60
388 T1	B cell	9/18/08	4	8 up-347 down	50	37 up-318 down	60

ISSN: 1743-422X