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Figure 2 | Virology Journal

Figure 2

From: Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages

Figure 2

Gel electrophoresis of patient 384 RT-PCR products using various sets of primers. For short time cultures and analyses, we have used cloned cell lines such as P3HR1 and CEM. This was for the purposes of reproducibility and economy. A. Primer sets 9.1a-9.2a (1st PCR) and 37up-318down (2nd PCR). Only 384 serum produced a positive band. CB designates Macrophages purified from cord blood. P3HR1-b and CB-b were supernatants collected at different times than P3HR1 and CB. B. Primer sets 9.1a-9.2a (1st PCR) and 10.1a-10.2 (2nd PCR). Only P3HR1 produced positive bands. C. Primer sets 8up-347down (1st PCR) and 37up-318down (2nd PCR). First PCR at 50°C. Only CEM produced positive bands. D. Primer sets 8up-347down (1st PCR) and 37up-318down (2nd PCR). First PCR at 55°C. Primary, P3HR1-b, and Cord blood macrophages produced positive bands.

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