Figure 1

Agarose gel electrophoresis showing amplicons obtained by the RT-Hemi-Nested-PCR for flavivirus from mosquitoes and larvae. A) Lanes 1 to 7 include amplification reaction products from larvae of Aedes albopictus from the City of Santos. Amplicon of ~628 bp compatible with DENV-3 in lanes 2, 3 and 4. B) Amplicon of ~472 bp, compatible with DENV-1, are shown in lanes 2 (Aedes aegypti of the City of Santos) and 3 (Haemagogus leucocelaenus from the County of Coribe). Line 4 shows an amplicon band of ~316 bp, compatible with DENV-2, obtained from Aedes aegypti captured in Foz do Iguaçu City. Lane 1 is a negative control and lane 5 is a positive control (DENV-3) of the RT-Hemi-Nested-PCR reaction.