Figure 3From: Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirusRestriction of RGNNV spread among medaka cells. (A) The cells (1.0-1.5 × 105) were inoculated with 106 TCID50 of RGNNV and cultured at 30°C. The CP and cell nucleus in the infected cells were detected by indirect immunofluorescence assay and DAPI staining, respectively, at the indicated period. Data represents the merged image of Alexa488-fluorescence and DAPI staining. (B) Rates for the infected cells against the total cells represented in Figures 1 and 3A were calculated and shown periodically.Back to article page