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Figure 5 | Virology Journal

Figure 5

From: Integrin αvβ5 is a primary receptor for adenovirus in CAR-negative cells

Figure 5

Ad5 binding to cells via integrin αvβ5 is high affinity. (A) Cells were incubated with various concentrations of Ad5 at 4°C for sufficient time for virus bound to reach equilibrium. Virus bound was measured as described in Figure 4. Y is the fractional occupancy, which is a ratio of virus bound to maximum virus bound. Data shown is the average of at least three independent experiments and error bars represent standard deviation. (B) Observed data (diamonds) was fit to calculated values (line) using the Langmuir Binding Isotherm (Eqn 1). Kd was determined by Microsoft Excel's Solver function using non-linear regression analysis to solve Eqn 1. A Kd of 1.4 × 10-10 M was calculated from the observed values.

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