Figure 2
From: Integrin αvβ5 is a primary receptor for adenovirus in CAR-negative cells
CAR expression levels. (A) Surface CAR levels were detected using flow cytometry. Cells were stained either with the monoclonal antibody RmcB which recognizes CAR (black line) or for control, only the secondary antibody, goat anti-mouse Alexa 488(grey line). The secondary only population was set to approximately 1% positive and used as the negative population for each cell line. The percentage of cells which are positive for CAR as well as the MFI is quantified and displayed in Table 1. Data shown is representative of at least two independent experiments. (B) CAR mRNA levels were determined using quantitative real-time PCR. CAR mRNA levels are compared to the control gene GapDH and data is presented as a ratio of CAR: GapDH. Data is the average of at least two independent experiments and error bars represent standard deviation.