Primer | Type | 5'pos | 3'pos | Length | Sequence |
---|---|---|---|---|---|
F3 | Forward outer | 1209 | 1228 | 20-nt | 5'-ggtttggcagaacagggata-3' |
B3 | Backward outer | 1406 | 1425 | 20-nt | 5'-gcccgtagagtactgggtta -3' |
FIP | Forward inner (F1c +F2) | 40-mer(F1c:22-nt, F2:18-nt) | 5'-ggccaaatcctccgagattcgg-cagggacctattggggca -3' | ||
BIP | Backward inner(B1c +B2) | 40-mer (B1c:20-nt, B2:20-nt) | 5'-caatccaccaccgcaggtgt-ccacttctggtgcacgtatt -3' | ||
LF | Loop Forward | 1259 | 1283 | 25-nt | 5'-TGGAATTTACCATCAGTCTTCGGTA-3' |
LB | Loop Backward | 1339 | 1362 | 24 | 5'-ATCAAGAATACACCAGTGCCTGCA-3' |