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Table 1 The primers used for LAMP

From: A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

Primer

Type

5'pos

3'pos

Length

Sequence

F3

Forward outer

1209

1228

20-nt

5'-ggtttggcagaacagggata-3'

B3

Backward outer

1406

1425

20-nt

5'-gcccgtagagtactgggtta -3'

FIP

Forward inner (F1c +F2)

  

40-mer(F1c:22-nt, F2:18-nt)

5'-ggccaaatcctccgagattcgg-cagggacctattggggca -3'

BIP

Backward inner(B1c +B2)

  

40-mer (B1c:20-nt, B2:20-nt)

5'-caatccaccaccgcaggtgt-ccacttctggtgcacgtatt -3'

LF

Loop Forward

1259

1283

25-nt

5'-TGGAATTTACCATCAGTCTTCGGTA-3'

LB

Loop Backward

1339

1362

24

5'-ATCAAGAATACACCAGTGCCTGCA-3'