Figure 1From: A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplificationDetermination of the optimal temperature and time of LAMP. (A) Determination of the optimal temperature. Lane M: DL-2000 marker; lanes 1: -, negative control; 2--7: LAMP carried out at 60, 61, 62, 63, 64 and 65°C, respectively. (B) Determination of the optimal time. lane M, DL-2000 marker; lanes 1: -, negative control; 2-7, LAMP carried out for 10, 20, 30, 40, 50 and 60 min, respectively. All the products were electrophoresed on a 2% agarose gels and stained with ethidium bromide.Back to article page