Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus strains with P[12] specificity

Table 1 The concentration of acrylamide affects VP4-gene coding assignment of group A equine rotavirus strains H-2, FI-14, and FI-23 bearing P[12] specificity.

Rotavirus			Species of origin	VP4-gene coding assignment in a PAGE gel containing acrylamide at indicated concentration
Strain [ref.]	P (VP4)type	G (VP7)type		5%	7.5%	10%	12%	15%
H-2 [18]	P[12]	G3	horse	4	4	3 or 4	3	3
FI-14 [19]	P[12]	G3	horse	ND^a	4	ND	3	ND
FI-23 [20]	P[12]	G14	horse	ND	4	ND	3	ND
H-1 [25]	P[7]	G5	horse	4	4	4	4	4
L338 [26]	P[18]	G13	horse	4	4	4	4	4
Wa [21]	P[8]	G1	human	4	4	4	4	4
DS-1 [21]	P[4]	G2	human	4	4	4	4	4
ST3 [22]	P[6]	G4	human	4	4	4	4	4
MMU18006 [23]	P[3]	G3	rhesus	4	4	4	4	4

ISSN: 1743-422X