Figure 2
From: Bovine ISG15: an antiviral and inducible protein in BIV infected fetal bovine lung cells
bISG15 plays a role in poly I:C induced-inhibition of BIV in FBL cells. a. The fluorescence microscopic image of 239T cells transfected with bISG15-EGFP expression plasmid, co-transfected with bISG15-EGFP expression plasmid and the scrambled siRNA, co-transfected with bISG15-EGFP expression plasmid and the bISG15siRNA respectively. b. bISG15siRNA can reverse the inhibition of BIV replication in FBL cells treated with poly I:C. The × axis indicates the four different experimental groups including the "BIV+ poly I:C+ scrambled siRNA" group, "BIV+ poly I:C + bISG15siRNA" group, "BIV + poly I:C" group and "BIV" group; the y axis indicates the relative luciferase activity folds. The bars show the relative luciferase activity of four groups, respectively. All the experiments were carried out more than 3 times, and the error bars are the standard deviation.