Figure 1

The replication of BIV was repressed in poly I:C treated FBL cells. a. BIV infection in FBL cells was measured quantitatively by BIV indicator cell line - BIVL cells. The × axis indicated the numbers of BIV infected cells; and the y axis indicated the relative luciferase activity folds. BIVL cells were plated at a density of 4 × 104 per well in 12-well plates. 1, 5, 10, 20, 50, 100 × 102 FBL cells were infected with BIV respectively, and normalized to the equal number (200 × 102) by adding naive FBL cells. The luciferase activity of adding only naive FBL cells was regarded as 1, and the relative luciferase activity folds of other groups were calculated. The relative folds were 1, 1.24, 2.73, 4.27, 7.23, 17.20, and 33.58. b. The replication of BIV in FBL cells indicated by relative luciferase activity in four experimental groups. The × axis indicates the four different experimental groups including the "NC" group (negative control), "BIV" group, "BIV + poly I:C" group, and "poly I:C" group; the y axis indicates the relative luciferase activity folds. The bars showed the relative luciferase activity folds of four groups respectively. All the experiments were carried out more than 3 times, the error bars are standard deviation (SD) from triplicate results.