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Table 1 Sensitivity and Specificity of the SYBR Green-based real-time RT-PCR using CHIKV nsP2 primer set.

From: Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection

Sample

Quantity

(PFU/ml)

Ct Value

Assay Results

Titrated seed CHIKV

   

Undiluted

105

9.11

Positive

Diluted 10-1

104

13.75

Positive

Diluted 10-2

103

17.89

Positive

Diluted 10-3

102

22.00

Positive

Diluted 10-4

101

26.45

Positive

Diluted 10-5

100

28.64

Positive

Other viruses tested in this assay

   

Ross River virus (Alphavirus)

104

40.00

Negative

Dengue virus Serotype 1 (Flavivirus)

104

40.00

Negative

Dengue virus Serotype 2 (Flavivirus)

105

40.00

Negative

Dengue virus Serotype 3 (Flavivirus)

104

40.00

Negative

Dengue virus Serotype 4 (Flavivirus)

104

40.00

Negative

Kunjin virus (Flavivirus)

105

40.00

Negative

West Nile virus (Sarafend, Flavivirus)

106

40.00

Negative

Enterovirus 71 (Piconavirus)

106

40.00

Negative

Echo virus type 7 (Piconavirus)

106

40.00

Negative