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Figure 3 | Virology Journal

Figure 3

From: Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection

Figure 3

Inhibitory assay of CHIKV infection using ribavirin. (A) Dosage dependent inhibition of CHIKV in Vero cells treated with different concentrations of ribavirin (62.5 μg/ml to 750 μg/ml). The cellular supernatants containing the infectious virus titers are determined by plaque assays (expressed as Log PFU/ml) as well as the one-step SYBR green-based quantitative RT-PCR (expressed as Ct value). (B) Correlation between the viral titers expressed as Log PFU/ml obtained from plaque assays and the one-step SYBR green-based quantitative RT-PCR are shown. Results from three independent experiments are included and statistical analysis is performed with a non-parametric (Spearman's correlation) test.

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Virology Journal

ISSN: 1743-422X