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Figure 2 | Virology Journal

Figure 2

From: Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection

Figure 2

Immunofluorescence assay (IFA) detection of viral antigen on CHIKV infected cells. Vero cells are infected with serially diluted CHIKV (expressed as PFU/ml) for 2 days and subjected to immunofluorescence staining with anti-alphavirus antibody. The CHIKV-infected cells are stained green and the cell nuclei are stained blue with the nuclear stain, DAPI. Mock-infected control and isotype control (cells are stained with secondary anti-mouse antibody conjugated with FITC) are included to ensure the specificity of the assay.

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Virology Journal

ISSN: 1743-422X