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Figure 4 | Virology Journal

Figure 4

From: Tomato leaf curl Kerala virus (ToLCKeV) AC3 protein forms a higher order oligomer and enhances ATPase activity of replication initiator protein (Rep/AC1)

Figure 4

AC3 modulates the ATPase activity of Rep. (a) Autoradiograph showing the ATPase activity of Rep in the absence and presence of AC3. AC3 increases the ATPase activity of Rep at low concentration (0.02-0.2 pM) by 50-80%. Composition of the proteins in the reaction mix is shown at the top of each lane in the autoradigraph. ATPase reaction was carried with a uniform concentration of Rep protein and varying concentrations of MBP-AC3 as denoted in the figure. MBP was used as a negative control. (b) Graphical representation of ATPase activity of Rep in the presence of MBP-AC3. ATPase activity in the reaction mix containing the Rep protein alone was arbitrarily assigned a value of 100% and activity in other lanes was calculated accordingly. Graph was plotted for the lanes 1-8 that correspond to the lanes of autoradiograph.

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Virology Journal

ISSN: 1743-422X