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Figure 3 | Virology Journal

Figure 3

From: Tomato leaf curl Kerala virus (ToLCKeV) AC3 protein forms a higher order oligomer and enhances ATPase activity of replication initiator protein (Rep/AC1)

Figure 3

GST pull-down assay for in vitro interaction of ToLCKeV GST-AC3 and MBP-AC1. Coomassie blue stained SDS-PAGE showing in vitro interaction between GST-AC3 and MBP-AC1. Fractions corresponding to 'input' (lanes 1-3) represent the total protein composition in each reaction mix. GST-AC3 bound proteins were thoroughly washed to remove the non-specifically interacting proteins. 'Bound' fractions (lanes 4-6) represent the proteins that were interacting with GST-AC3. Presence of AC1 in the bound fraction (lane 6) indicates its interaction with AC3.

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Virology Journal

ISSN: 1743-422X