Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Tomato leaf curl Kerala virus (ToLCKeV) AC3 protein forms a higher order oligomer and enhances ATPase activity of replication initiator protein (Rep/AC1)

Figure 2

ToLCKeV AC3 forms a higher order oligomer. (a) Western blotting of GST pull-down assay using poly-clonal MBP-AC3 antibodies. Fractions corresponding to 'input' represent the protein composition of the total reaction mix for protein-protein interactions. Fractions corresponding to 'bound' represent the proteins that are interacting with GST-AC3 bound to glutathione sepharose. Presence of MBP-AC3 in the bound fraction indicates the formation of oligomer. (b) [i] Protein distribution pattern for the MBP-AC3 after sucrose gradient ultracentrifugation was visualised by Coomassie blue staining. MBP-AC3 forms a faint peak at 11 th fraction and a prominent peak at 32 nd fraction as indicated by 'V'. Arrows indicate the peak formation of molecular weight standard proteins: Aldolase (158 kDa) at 17 th fraction, Ferritin (449 kDa) at 26 th fraction and Thyroglobulin (669 kDa) at 30 th fraction. [ii] MBP (43 kDa) does not form an oligomer and peaks in the 5 th fraction. (c) Gel filtration with Superdex-200 5/150 column shows the elution of various proteins. MBP-AC3 elutes between the Dextran (2000 kDa) and Thyroglobulin (669 kDa).

Back to article page

Virology Journal

ISSN: 1743-422X