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Figure 3 | Virology Journal

Figure 3

From: Multiplex Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) for diagnosis of natural infection with canine distemper virus

Figure 3

Sequence alignment of partial F gene. (A)The F gene nucleotide sequences, including the intergenic region between M and F gene (M-F UTR), of field strains from Taiwan and three commercial vaccine strains were analysed. The numbering starts at the first amino acid of the M-F UTR. Only amino acids that differ from the majority sequence are shown. Identical residues are represented by dots. The substitution of the AAA present in the vaccine strains, which was used to design the differentiating primers for ARMS-PCR, is indicated by a square box. (B) The region consisting of TCG motif, located 35 nucleotide downstream from the start codon (ATG) was comparatively aligned with various CDV lineages: Asia-1 strain; TW-KS2, TW-TP1, TW-KL1, HeB-07, JL-07, NM, ZD01, BS0610 (GenBank: EU192013, EU191985, EU191988, EU327874, EU327875, EF596903, EF596904, EU934234), Asia-2 strain; 007 Lm (GenBank: AB474397), Europe strain; 5047/91, R252/72, Rockborn, X65509 (GenBank: AF026240, AF026243, AF026244, X65509), America strain; A75/17, 01-2689, 5804, 5804P, 00-2601 (GenBank: AF164967, AY649416, AY386315, AY386 316, AY443350), and Vaccine strain; Vaccine X, Snyder Hill and Onderstepoort (GenBank: EU072198, GU138403, AF305419). Omitted sequences are represented by dots.

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Virology Journal

ISSN: 1743-422X